Detection and differentiation of phytoplasmas in Australia

Detection and differentiation of phytoplasmas in Australia

Title
Publication TypeJournal Article
Year of Publication1997
AuthorsDavis, R, Schneider, BL, Gibb, KS
JournalAustralian Journal of Agricultural Research
Volume48
Issue5
Pagination535 - 544
Date Published01/01/97
ISSN0004-9409
Abstract

In a polymerase chain reaction (PCR) diagnostic test, phytoplasma (formerly known as plant-pathogenic mycoplasma-like organism or MLO) ribosomal DNA was detected in total DNA extracts prepared from 56 out of 63 plants collected from geographically diverse locations across Australia. The list of phytoplasma hosts consisted of 38 di®erent species in 16 di®erent families. Restriction site analysis of the PCR-ampli¯ed DNA accessions was used to divide the phytoplasmas into 2 groups. The majority of the tomato big bud group and sweet potato little leaf group phytoplasmas were closely related to a phytoplasma originally obtained from Crotalaria in Thailand, which is a member of the faba bean phyllody strain cluster. In contrast, phytoplasmas associated with Australian grapevine yellows and papaya dieback were most similar to members of the aster yellows strain cluster. Twelve phytoplasmas were compared by Southern blot hybridisation with DNA cloned from the sweet potato little leaf phytoplasma strain V4. The restriction fragment length polymorphism pattern of all phytoplasmas compared was identical except for 2 sweet potato little leaf phytoplasmas.

URLhttp://espace.cdu.edu.au/view/cdu:851

Publications

Citation and export

Davis, R., Schneider, B. L. & Gibb, K. S. Detection and differentiation of phytoplasmas in Australia. Australian Journal of Agricultural Research 48, 535 - 544 (1997).

RIEL Seminar Series

Mila Bristow is a post-doc at CDU’s Research Institute for the Environment and Livelihoods investigating the impact of...

Fri, 02/05/2014 - 10:00 to 11:00 Charles Darwin University (Building Red 1.3.01)

RIEL Headlines

  • Thu, 05/12/2013

    In an intensive effort to better understand the role of feral cats in native mammal decline, two cat-exclosure fences have been built in Kakadu National Park.

  • Tue, 03/12/2013

    An innovative new database compiling information about crocodilian attacks world-wide could help with future conservation efforts of the species’.

Pages

Jump to NRBL themeJump to CMEM themeJump to FEM themeJump to SMWC themeJump to TRF themeJump to RIEL home

Innovative Research University

© 2011-2013 Charles Darwin University
Research Institute for the Environment and Livelihoods
Privacy Policy
CRICOS Provider No. 00300K | RTO Provider No. 0373

Phone (+61) 8 8946 6413
Email riel@cdu.edu.au